Standard operating procedures for PCR reaction tube test

1、 Character

The PCR reaction tube was colorless and transparent.

2、 Negative reference

PCR system solution was prepared by using the sample to be tested, other qualified raw materials and large package mixed enzyme solution. All negative reference samples were negative, and the coincidence rate was 100%.

3、 Detection limit

The PCR system was prepared with the sample to be tested, other qualified raw materials and large package mixed enzyme solution. The low detection limit of Zui was 1000 copies / ml.

4、 Positive control

The PCR system was prepared by using the sample to be tested, other qualified raw materials and large package mixed enzyme solution. The positive control was detected. The positive control was found to be positive, with CT ≤ 25, and the amplification curve had an obvious exponential growth period.